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KMID : 0363420100240020126
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Journal of Korean Oriental Pediatrics
2010 Volume.24 No. 2 p.126 ~ p.136
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The Antiallergic and Antioxidative Effects of Chaenomeles Sinensis (CS) in RBL 2H3 Cells
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Lee Seung-Yeon
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Abstract
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Objectives: The purpose of this study was to investigate the antiallergic and antioxidative effects of Chaenomeles Sinensis (CS).
Methods: CS pretreatments inhibited anti-DNP IgE in RBL-2H3 mast cells for an hour. we measured cell viability, -hexosaminidase release, IL-4, TNF- secretion, and IL-4, TNF- mRNA expression CS pretreatments inhibited DNP-HSA() for ten minutes, we measured Dicholrodihydrofluorescein(DCF) and DPPH radical-scavenging activity in 0.5 mM 1,1-diphenyl-2-picrydrazyl(DPPH) radical solution, 0.1ml, 99% ethanol 0.8ml, and CS 0.1 ml mixed solution.
Results: 0, 1, 2, 3, 4, 5 mg/ml CS treatments were not affect on cell viability and inhibited b-hexosaminidase release, IL-4, TNF- secretion, CS treatments also decreased IL-4, TNF- mRNA expression in RBL-2H3 cells. CS treatments inhibited reactive oxygen species(ROS) and DPPH radical-scavenging activity.
Conclusions: These results suggest that CS may be useful for the prevention or treatment of allergic disease
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KEYWORD
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Chaenomeles Sinensis, β -hexosaminidase, IL-4, TNF-α , ROS, DPPH radical-scavenging activity
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